博士生导师，中山大学百人计划引进人才
研究方向
1. 噬菌体的组装以及与宿主的相互作用研究
2. 基于噬菌体的应用探索
工作经历
2021/03-至今中山大学公共卫生学院（深圳），教授
2019/11-2021/02加州大学伯克利分校，博士后
2016/01-2019/11普渡大学，博士后
教育经历
2010/09-2015/12中国科学院生物物理研究所，生物化学与分子生物学，理学博士
2006/09-2010/07华中科技大学，生物科学专业，理学学士
研究简介
噬菌体被认为是自然界中数量最多的一类病毒，具有杀灭细菌的能力。本课题组采用冷冻电镜技术、生物化学与分子生物学以及细胞生物学等多种方法，深入探索噬菌体的组装过程及其与宿主之间的相互作用机制。基于对噬菌体组装及宿主相互作用机制的深入理解，我们进一步进行以下基于噬菌体的应用探索：1）通过对天然噬菌体的改造与优化，开发新型噬菌体生物制剂，以应对耐药菌感染，作为抗生素的替代选择；2）对噬菌体病毒衣壳进行改造，使其能够容纳外源基因，从而开发可用于肿瘤治疗及核酸疫苗等领域的基因递送载体；3）改造具有抗菌潜力的噬菌体编码蛋白，以研发新型抗菌药物。已在国际知名期刊上发表多篇SCI文章，包括《Science》、《Nature Structural & Molecular Biology》、《Nature Communications》、《PNAS》、《eLife》、《Structure》、《Journal of Molecular Biology》、《Journal of Virology》等。
承担课题及科研项目
国家青年人才项目基金（2021-2024），主持
国家自然科学基金面上项目（2024-2027），主持
中山大学“百人计划”启动项目，项目负责人
深圳市基础研究面上项目（2024-2027），主持
深圳市医学研究专项资金项目（生物技术研究）（2024-2025），主持
国家重点研发专项（2021-2024），参与
代表性文章
24. Zhao, X.#, Wang, A.#, Wang, Y.#, Kang, Y.#, Shao, Q., Li, L., Zheng, Y., Hu, H., Li, X., Fan, H., Cai, C.*, Liu, B.*, & Fang, Q.*Capsid structure of phage SPO1 reveals novel minor capsid proteins and insights into capsid stabilization. Structure. (2025).
23. Van Etten, J.*, Agarkova, I., Dunigan, D., Shao, Q., Fang, Q. Emerging structure of chlorovirus PBCV-1. Virology. (2025).
22. Cai, C.#, Wang, Y.#, Liu, Y.#, Shao, Q., Wang, A., Li, L.,Zheng, Y., Zhang, T., Luo, Z., Yang, C., & Fang, Q*. Structures of a T1-like siphophage reveal capsid stabilization mechanisms and high structural similarities with a myophage. Structure. (2025).
21. Li, X.#, Shao, Q.#, Li, L., Xie, L., Ruan, Z., Fang, Q.* Cryo-EM Reveals Structural Diversity in Prolate-headed Mycobacteriophage Mycofy1. Journal of Molecular Biology, (2025).
20. Guo, M.#, Wang, A.#, Zheng, Y.#, Liu, C.#, Shao, Q., Deng, Y., Li, L., Wang, Y., Wang, X., Shen, Y., Qian, J.*, Zhou, X.*, Fang, Q*. Cryo-EM structures of a Xanthomonas phage: Insights into viral architecture and implications for the model phage HK97. Structure, (2025).
19. Yang, Y.#, Shao, Q.#, Guo, M.#, Han, L.#, Zhao, X., Wang, A., Li X., Wang B., Pan JA., Chen Z., Fokine A., Sun L.*, & Fang, Q*.. Capsid structure of bacteriophage ΦKZ provides insights into assembly and stabilization of jumbo phages. Nature Communications, 15(1), 6551(2024).
18. Ruan, Z.#, Shao, Q.#, Song, Y.#, Hu, B.#, Fan, Z., Wei, H., Liu, Y., Wang, F.*, & Fang, Q*. Near-atomic structures of RHDV reveal insights into capsid assembly and different conformations between mature virion and VLP. Journal of Virology, 98(11), e01275-24(2024).
17. Tang, K., Sun, Q., Zeng, J., Tang, J., Cheng, P., Qiu, Z., Long, H., Chen, Y., Zhang, C., Wei, J., Qiu, X., Jiang, G., Fang, Q., Sun, L., Sun, C., Du, X. Network-based approach for drug repurposing against mpox. International Journal of Biological Macromolecules, (2024).
16. Ferro, L.S.#, Fang, Q.*#, Eshun-Wilson, L.#, Fernandes, J.#, Jack, A., Farrell, D.P., Golcuk, M., Huijben, T., Costa, K., Gur, M., DiMaio, F., Nogales, E.*, & Yildiz, A*. Structural and functional insight into regulation of kinesin-1 by microtubule- associated protein MAP7. Science (New York, N.Y.), 375(6578), 326-331(2022).
15. Shao, Q., Agarkova, I. V., Noel, E. A., Dunigan, D. D., Liu, Y., Wang, A., Guo, M., Xie, L., Zhao, X., Rossmann, M. G., Van Etten, J. L.*, Klose, T.*, & Fang, Q*.. Near-atomic, non-icosahedrally averaged structure of giant virus Paramecium bursaria chlorella virus 1. Nat Commun 13(1), 6476(2022).
14.Fang, Q.#, Tang, W. C.#, Fokine, A.#, Mahalingam, M., Shao, Q., Rossmann, M. G., & Rao, V. B*. Structures of a large prolate virus capsid in unexpanded and expanded states generate insights into the icosahedral virus assembly. Proc Natl Acad Sci U S A, 119(40), e2203272119(2022).
13. Khare, B., Klose, T., Fang, Q., Rossmann, M. G., & Kuhn, R. J. Structure of Usutu virus SAAR-1776 displays fusion loop asymmetry. Proc Natl Acad Sci USA 34, 118 (2021).
12. Du L., Xie Y., Zheng K., Wang N., Gao M., Yu T., Cao L., Shao Q., Zou Y., Xia W., Fang Q., Zhao B., Guo D., Peng X., Pan JA. Oxidative stress transforms 3CLpro into an insoluble and more active form to promote SARS-CoV-2 replication. Redox Biol 48, 102199 (2021).
11. Herbst D.A., Esbin M.N., Louder R.K., Dugast-Darzacq C., Dailey G.M., Fang Q., Darzacq X., Tjian R., Nogales E. Structure of the human SAGA coactivator complex. Nat Struct Mol Biol 28, 989-996 (2021).
10. Rao, V.B., Fokine, A. & Fang, Q. The remarkable viral portal vertex: structure and a plausible model for mechanism. Curr Opin Virol 51, 65-73 (2021).
9.Fang, Q., Tang, W.C., Tao, P., Mahalingam, M., Fokine, A., Rossmann, M.G., Rao, V.B. Structural morphing in a symmetry-mismatched viral vertex. Nat Commun 1, 1713 (2020).
8. Sun, Y., Watters, K., Hill, M.G., Fang, Q., Liu, Y., Kuhn, R.J., Klose, T., Rossmann, M.G., and Palmenberg, A.C. Cryo-EM structure of rhinovirus C15a bound to its cadherin-related protein 3 receptor. Proc. Natl Acad. Sci. USA (2020).
7.Fang, Q.#, Zhu, D.#, Agarkova, I., Adhikari, J., Klose, T., Liu, Y., Chen, Z., Sun, Y., Gross, M.L., Van Etten, J.L., Zhang, X.*, Rossmann, M.G.*. Near-atomic structure of a giant virus. Nat Commun 10, 388 (2019).
6. Ananthaswamy, N.#, Fang, Q.#, AlSalmi, W.#, Jain, S.#, Chen, Z., Klose, T., Sun, Y., Liu, Y., Mahalingam, M., Chand, S., Tovanabutra, S., Robb, M.L., Rossmann, M.G.*, Rao, V.B.*. A sequestered fusion peptide in the structure of an HIV-1 transmitted/founder envelope trimer. Nat Commun 10, 873 (2019).
5. Zhang, Z., Wang, X., Xin, J., Ding, Z., Liu, S., Fang, Q., Yang, N., Xu, R.M., and Cai, G.. Architecture of SWI/SNF chromatin remodeling complex. Protein Cell 9, 1045-1049 (2018).
4. Zhu, D., Wang, X., Fang, Q., Van Etten, J.L., Rossmann, M.G., Rao, Z., and Zhang, X. Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions. Nat Commun 9, 1552 (2018).
3. Fang, Q.#, Chen, P.#, Wang, M., Fang, J., Yang, N., Li, G.*, and Xu, R.M.*. Human cytomegalovirus IE1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome. Elife 5 (2016).
2. Li, Y., Zhang, L., Liu, T., Chai, C., Fang, Q., Wu, H., Agudelo Garcia, P. A., Han, Z., Zong, S., Yu, Y., Zhang, X., Parthun, M. R., Chai, J., Xu, R. M., Yang, M. Hat2p recognizes the histone H3 tail to specify the acetylation of the newly synthesized H3/H4 heterodimer by the Hat1p/Hat2p complex. Genes Dev 28(11): 1217-1227 (2014).
1. Yang, D.#, Fang, Q.#, Wang, M.#, Ren, R., Wang, H., He, M., Sun, Y., Yang, N.*, Xu, R.M.*. Nalpha-acetylated Sir3 stabilizes the conformation of a nucleosome-binding loop in the BAH domain. Nat Struct Mol Biol 20, 1116-8 (2013).
招生信息
方强林教授团队热忱欢迎热爱科学研究的优秀学生报考硕士生和博士生（2-4人/年）；接收本科生进行科研培训实习（1-2人）；招收博士后以及专职科研人员（特聘研究员、特聘副研究员、助理研究员等），业绩优秀者聘期结束后可直接申报中山大学副教授/教授岗位。欢迎对病毒学、噬菌体结构生物学、冷冻电镜技术、蛋白质设计等感兴趣的有志之士加入本实验室！请有意者将个人简历通过电子邮件发至：fangqlin3@mail.sysu.edu.cn，来信请注明申请方向及职位。
